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AF/LE Purified Anti-Mouse CD45.2 Antibody[104.2]

AF/LE Purified Anti-Mouse CD45.2 Antibody[104.2]

Item no.	E-AB-F11220-25mg
Manufacturer	Elabscience
Amount	25 mg
Quantity options	1 mg 100 mg 100 ug 25 mg 25 ug 50 ug 500 ug 5 mg
Category	Oncology Neuroscience Neural Lineage Markers Antibodies By Cell Type Carcinoma Hematology Leukemia Flow Cytometry Immunohistochemistry Primary Antibodies Progenitor Markers
Type	Antibody Monoclonal
Applications	FC, Blocking
Clone	104.2
Specific against	Mouse (Murine, Mus musculus)
Host	Mouse
Isotype	IgG2a Kappa
Conjugate/Tag	Unconjugated
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Ly-5.2, LCA,
Shipping Condition	Cool pack
Hazard information	Comply with cargo safety certificate
Available
Manufacturer - Applications	FCM, Block
Manufacturer - Category	Flow Cytometry Antibodies
Manufacturer - Isotype	Mouse IgG2a, κ
Manufacturer - Targets	CD45.2
Manufacturer - Conjugate / Tag	None (AF/LE)
Shipping Temperature	Ice bag
Storage Conditions	Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Background	CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Concentration	≥1 mg/mL
Buffer	Sterile PBS. < 1.0 EU per mg of the antibody as determined by the LAL method.
Abbreviation	CD45.2
Clonality	Monoclonal
Usage	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10^6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.