« Back
Home /
AF/LE Purified Anti-Human CD20 Antibody[2H7]

AF/LE Purified Anti-Human CD20 Antibody[2H7]

Item no.	E-AB-F12120-100mg
Manufacturer	Elabscience
Amount	100 mg
Quantity options	1 mg 100 mg 100 ug 25 mg 25 ug 50 ug 500 ug 5 mg
Category	Oncology Antibodies By Cell Type Hematology Leukemia Lymphoma Myeloma Flow Cytometry Immunohistochemistry Immunoprecipitation Primary Antibodies By Organ Bladder Cancer Gastric Cancer
Type	Antibody Monoclonal
Applications	FC
Clone	2H7
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2b Kappa
Conjugate/Tag	Unconjugated
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	Bp35, Leukocyte surface antigen Leu-16, MS4A1, B-lymphocyte surface antigen B1,
Shipping Condition	Cool pack
Hazard information	Comply with cargo safety certificate
Available
Manufacturer - Applications	FCM, Depletion
Manufacturer - Category	Flow Cytometry Antibodies
Manufacturer - Isotype	Mouse IgG2b, κ
Manufacturer - Targets	CD20
Manufacturer - Conjugate / Tag	None (AF/LE)
Shipping Temperature	Ice bag
Storage Conditions	Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Background	CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.
Concentration	≥1 mg/mL
Buffer	Sterile PBS. < 1.0 EU per mg of the antibody as determined by the LAL method.
Abbreviation	CD20
Clonality	Monoclonal
Usage	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 10^6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.