Anti-Mouse CD1d (CD1.1) - Purified <i>in vivo </i>GOLD™ Functional Grade - 50 mg

B, ELISA, FC, IP, WB

2 - 8°C Wet Ice

CD1d is a non-classical major histocompatibility complex (MHC) molecule that presentsglycolipid instead of peptide antigens1 . CD1d is non-MHC-encoded, heavily glycosylated, and asurface protein associated with β2-microglobulin2 . CD1d is present on invariant natural killer(iNKT) cells, where it elicits an immune response upon ligation with the T cell receptor andagonist α-galactosylceramide (α-GalCer )1. iNKT cells activated by α-GalCer secrete cytokinesand undergo proliferation. Additionally, α-GalCer can induce anti-tumor immunity. CD1d canalso interact with the iNKT agonist β-mannosylceramide (β-ManCer).20H2 was generated by immunizing male Wistar rats with C57BL/6 thymocytes, boosting threetimes with a RBL-CD1.1-transfected rat cell line, injecting once with BALB/c thymocytes, andfinally injecting with CR1-CD1.1 cells2 . The resulting spleen cells were fused with Sp2/0 cellsfor hybridoma production. 20H2 cross-reacts with CD1.2-transfected cells and binds to anepitope in the vicinity of the CD1d antigen-binding groove.20H2 blocks T cell recognition of CD1d2 and can be used to block β-ManCer and α-GalCeragonistic activity1 . Blocking with 20H2 may also attenuate non-allergic asthma3 and amelioratesome aspects of neurodegenerative pathology in mouse4.

≥ 5.0 mg/ml

CD1d (CD1.1) is expressed on type I natural killer T cells also known asinvariant NKT cells. CD1d is expressed by dendritic cells, macrophages, and B cells, particularlysplenic marginal zone B cells.