Anti-Human CD45 (Clone 2B11) - PE - 50 µg

CD45

CD45 is a 180-240 kDa glycoprotein member of the protein tyrosine phosphatase (PTP) familyknown for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vitalregulators of T- and B-cell antigen receptor signaling. It functions through its extracellulardomain or through its cytoplasmic domain and serves as a negative regulator of cytokine receptorsignaling via JAK kinase suppression. The large extracellular domain is highly glycosylated, andits multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoformsshow cell-type and differentiation-stage specific expression that can be used as markers thatidentify and distinguish between different types of immune cells. CD45R is an isoform of CD45with a molecular weight of 220 kDa. CD45R contains all three possible exons (A, B, and C), making it the longest protein generated from alternative splicing with a migration at 200 kDawhen isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kDa, hence the name B220. Notably, B220 expression isnot restricted to B cells and may be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytesexpress CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform. 2B11 was originally generated as against leukocyte common antigen1. Isolated neoplastic cellsfrom T cell lymphoma were used as and immunogen for 2B11. 2B11 stains most T cell and Bcell lymphomas but not Reed-Sternberg cells or variants of Hodgkin's disease2. 2B11 also stainsosteoclasts3 and has been tested in the pathology setting and can be used to distinguishneoplasms4-9.

0.2 mg/ml

Various cell dependent isoforms of CD45 are expressed on B cells, T cells, subsets of dendritic cells and other antigen-presenting cells, monocytes and mast cells.