Anti-Human/Mouse/Rat Glycophorin A - Dylight® 488

FC, IF, IHC, IHC FFPE, WB

Glycophorin A

Glycophorins are transmembrane proteins of red blood cells (RBC)1 . There are four glycophorin encoding genes (A, B, C, and D), and a fifth pseudogene (E), which has no protein product.Glycophorin A, B, and E are tandemly arranged repeats on chromosome 4 and are prone torearrangements that lead to rare blood groups. Glycophorin A and B carry MN and Ss blood group antigens.Glycophorin A is a type I glycoprotein carrying sialylated O- and N-glycans, and most of the O-glycans are NeuAcα2-3Galβ1-3(NeuAcα2-6)GaINAc-tetrasaccharide chains linked to serine orthreonine residues 2 . The abundance of glycophorin A on the surface of RBCs is thought to createa negatively charged cell surface of complex glycans that allows for RBC circulation withoutsticking to other cells or blood vessel walls1 .Glycophorin A binds CD170 3 (Siglec 5, which mediates sialic-acid dependent binding to cells4), influenza virus, and EBA-175, the erythrocyte binding antigen of Plasmodium falciparum, theparasite responsible for malaria in Africa 2 . Babesia divergens, which like Plasmodium is aparasite in the Apicomplexa phylum, also uses glycophorins A and B to enter RBCs. Otherpathogens that bind to glycophorin A on the surface of RBCs include: some strains ofEscherichia coli, reoviruses (a group of viruses that includes rotavirus), encephalomyocarditis virus, and hepatitis A 2 . Glycophorin A also interacts with influenza virus but because influenza virus cannot replicate in anucleated RBCs glycophorin A might be acting as a decoy receptor to divert infection away from other tissues.JC159 was generated using splenic hairy cell leukemia cells as immunogen and reacts with aformalin-fixed, paraffin-embedded resistant epitope, likely between amino acids 27 and 40, onthe extracellular domain5.

0.2 mg/ml

Glycophorin A (CD235a) is expressed on hematopoietic stem cells, erythroid cells, and erythrocytes. Erythroleukemia can express glycophorin A on neoplasticerythroblasts.