Anti-Mouse TIM-4 – Purified in vivo GOLD™ Functional Grade - 1 mg

B, IF

2 - 8°C Wet Ice

The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cellsurface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediatedimmunity1. TIM-4, which is preferentially expressed on macrophages and dendritic cells, is thenatural ligand of TIM-1, and this binding leads to T-cell expansion and cytokine production.Unlike other members of the TIM family, TIM-4 lacks a putative tyrosine phosphorylation signalsequence in its intracellular domain. The TIM-4 gene maps to a locus associated withpredisposition to asthma in both mice and humans and with its connection to TIM-1-triggeredTh2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma.RMT4-53 was generated by linking the extracellular domains of TIM-4 (aa 1-288) to the Fcportion of mouse IgG2a2. This protein product was then used to immunize Sprague Dawley rats.Subsequently, LN cells were fused with P3U1 myeloma cells. RMT4-53 reacts with TIM-4/NRKcells but not with parental NRK or other TIM family members.TIM-4 blockade has been investigated for the treatment of cancer3, 4 and allograft rejection2, 5 using anti-TIM-4 clone RMT4-53. Additionally, RMT4-53 blockade of TIM-4 leads to increased induction of iTregs from naïve CD4+ T cells2. Blockade with RMT4-53 has also been investigated in liver ischemia-reperfusion injury6, 7.

≥ 5.0 mg/ml

TIM-4 is expressed by antigen-presenting cells of the lymphoid lineage, preferentially by maturedendritic cells and macrophages.