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Cd3e Antibody

Cd3e Antibody

Item no.	OAEE00181
Manufacturer	AVIVA Systems Biology
Amount	0.1 mg (C100)
Category	Oncology Neuroscience Molecular Targets for Neuroscience Antibodies By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Adenocarcinoma Flow Cytometry Immunohistochemistry Immunoprecipitation Immunocytochemistry Primary Antibodies Functional Assays
Type	Antibody Monoclonal
Applications	FC, IP, FA
Clone	MEM-57
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG2a
Purity	> 95% (by SDS-PAGE)
Citations	Horejsi V. et al.: Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol. (Praha) 34, 23 (1988). And other., Dave VP, Cao Z, Browne C, Alarcon B, Fernandez-Miguel G, Lafaille J, de la Hera A, Tonegawa S, Kappes DJ: CD3 delta deficiency arrests development of the alpha beta but not the gamma delta T cell lineage. EMBO J. 1997 Mar 17;16(6):1360-70., Panyi G, BagdÃ¡ny M, BodnÃ¡r A, VÃ¡mosi G, Szentesi G, Jenei A, MÃ¡tyus L, Varga S, Waldmann TA, GÃ¡spar R, Damjanovich S: Colocalization and nonrandom distribution of Kv1.3 potassium channels and CD3 molecules in the plasma membrane of human T lymphocytes. Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2592-7. Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, SchÃ¼tz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84. BrdickovÃ¡ N, Brdicka T, AngelisovÃ¡ P, HorvÃ¡th O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, HorejsÃ V: LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62. Soucek J, Chudomel V, HrubÃ¡ A, LindnerovÃ¡ G: Induction of NK and LAK activities in human lymphocyte culture by a cytosol fraction from leukemic myeloblasts and by monoclonal antibody CD 3. Neoplasma. 1991;38(1):33-41. Soucek J, Hilgert I, BudovÃ¡ I, LindnerovÃ¡ G: Augmentation of NK cell activity and proliferation in cultured lymphocytes ofleukemic patients by monoclonal antibodies CD3 and interleukin-2. Neoplasma. 1994;41(2):75-81. Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989); p. 293. Hilgert I. et al.: Therapeutic in vivo use of the A1-CD3 monoclonal antibody. Transplantation 55, 435 (1993). Leukocyte Typing III., McMichael M.J. et al. (Eds.), Oxford University Press (1987); p.611. Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279(28):28827-30. AlarcÃ³n B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7(5):490-5. Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb;24(2):133-9.
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Alias	CD3e antigen,epsilon polypeptide (TiT3 complex);CD3e molecule,epsilon (CD3-TCR complex);CD3-epsilon;IMD18;T3E;T-cell antigen receptor complex,epsilon subunit of T3;T-cell surface antigen T3/Leu-4 epsilon chain;T-cell surface glycoprotein CD3 epsilon chain;TCRE.
Shipping Condition	Cool pack
Available
Manufacturer - Type	Monoclonal Antibody
Manufacturer - Applications	Flow cytometry\|Functional assay\|Immunoprecipitation\|Mass cytometry
Manufacturer - Category	Root Catalog/Products/Monoclonal Antibodies, Root Catalog/Products/Primary Antibodies
Shipping Temperature	Wet Ice
Gene symbol	CD3E
Gene Fullname	CD3e molecule
Product format	Phosphate buffered saline (PBS), pH 7.4
Reconstitution and storage	2°C to 8°C
Description of target	Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell developement. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).
Nucleotide accession_num	NM_007648.4; XM_006509966.2
Protein accession_num	NP_031674.1; XP_006510029.1
Protein name	T-cell surface glycoprotein CD3 epsilon chain
Clonality	Monoclonal
Purification	Purified
Immunogen	Human thymocytes and T lymphocytes.
Manufacturer - Specificity	The antibody MEM-57 reacts with gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. HLDA IV.; WS Code T 96
Formulation	Low endotoxin
Concentration	1 mg/ml
Storage Buffer	Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg (C100)

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