Comparison

PPP1R12A Antibody (Phospho-Thr853)

Item no. OASG04799
Manufacturer AVIVA Systems Biology
Amount 100 ul
Category
Type Antibody Polyclonal
Format Liquid
Applications WB, IHC
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Rabbit
Isotype IgG
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias MBS,GUBS,M130,MYPT1
Shipping Condition Cool pack
Available
Manufacturer - Type
Polyclonal Antibody
Manufacturer - Category
Root Catalog/Products/Polyclonal Antibodies, Root Catalog/Products/Polyclonal Antibodies/Phospho Specific, Root Catalog/Products/Primary Antibodies
Manufacturer - Targets
Phosphorylated by CIT (Rho-associated kinase). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK; may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates PPP1R12A.
Shipping Temperature
Wet Ice
Molecular Weight
130 kDa
Manufacturer - Application Additional Information
WB: 1:500-1:2, 000 IHC: 1:100-1:300ELISA: 1:5, 000Optimal dilutions should be determined by the end user.
Gene symbol
PPP1R12A
Gene Fullname
protein phosphatase 1, regulatory subunit 12A
Product format
Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Reconstitution and storage
Store at -20°C, and avoid repeat freeze-thaw cycles.
Description of target
MYPT1 is a protein encoded by the PPP1R12A gene which is approximately 115, 2 kDa. MYPT1 is localised to the cytoplasm. It is involved in the activation of cAMP-dependent PKA, beta-adrenergic signalling, vascular smooth muscle contraction and actin nucleation by ARP-WASP complex. It regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho and is also a key regulator of protein phosphatase 1C. MYPT1 is expressed in striated muscles, specifically in type 2a fibres. Mutations in the PPP1R12A gene may result in infratentorial cancer and medullomyoblastoma. STJ90822 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous MYPT1 protein which only binds about T853 when T853 is phosphorylated.
Clonality
Polyclonal
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
Synthesized peptide derived between 590-670aa from human MYPT1 around the phosphorylation site of T853.
Manufacturer - Specificity
Phospho-MYPT1 (T853) Polyclonal Antibody detects endogenous levels of MYPT1 protein only when phosphorylated at T853.
Concentration
1 mg/ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ul
Available: In stock
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