Comparison

POLR2A Antibody

Item no. PRS-13-946-100uL
Manufacturer ProSci
Amount 100 uL
Quantity options 100 uL
Category
Type Antibody Primary
Format Liquid
Applications WB, IF, IP, IHC, CHIP
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Pig (Porcine, Sus scrofa domesticus)
Host Rabbit
Isotype IgG
Conjugate/Tag Unconjugated
NCBI POLR2A
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias POLR2A,HRPB220,HsRPB1,POLR2,RpIILS,RPOL2,RNA Polymerase II,RPO2,POLRA,RNA polymerase II subunit B1,RPB1,RPBh1
Shipping Condition Cool pack
Available
Specificity Human,Mouse,Rat,Pig
Manufacturer - Type
Primary Antibodies
Shipping Temperature
Blue Ice
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Molecular Weight
Observed: 250kDa
Manufacturer - Research Area
Neuroscience
Background
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
By Type
Neuroscience
Modifications
None
Purification
Affinity purification
Positive Control 1
HeLa
Positive Control 2
BT-474
Positive Control 3
H460
Positive Control 4
Jurkat
Positive Control 5
Mouse testis
Positive Control 6
Mouse brain
Concentration
batch dependent
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Note
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
ChIP: 1:20 - 1:50
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A (NP_000928.1).
Clonality
Polyclonal
NCBI Official Name
polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
NCBI Organism
Homo sapiens
User Note
Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 uL
Available: In stock
available

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