Glial Fibrillary Acidic Protein (GFAP)

Antibodies / Antibodies-Growth Factors, Cytokines

-20°C

Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol.

Purified porcine spinal cord GFAP

Glial fibrillary acidic protein (GFAP) is strongly and specifically expressed in astrocytes, Bergmann glia, certain other glia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. GFAP expression is also seen in developing neural stem cells and GFAP levels may greatly increase in regions of CNS injury or disease. The formation of a GFAP rich “glial scar” following CNS injury may be one reason why reconnection of severed processes is relatively inefficient in adults. Point mutations in the GFAP gene are causative of Alexander disease (5). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes. Some interest has recently been focused on GFAP as a protein released into blood and CSF following traumatic brain injury, stroke and other CNS compromises (6, 7). Measurement of the levels of blood or CSF GFAP may give information about patient presentation, progress, response to therapy or outcome.

Useful for visualizing glia and monitoring developmental, disease and damage related CNS alterations.

Applications:
Suitable for use in Immunofluorescence/Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunofluorescence (IC): 1:1000
Immunohistochemistry: 1:1000
Western Blot: 1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.