ACE2 (SARS Receptor, Angiotensin-converting Enzyme 2, ACEH)

Antibodies / Antibodies-Infectious Disease SARS

-20°C

Supplied as a liquid in PBS, pH 7.4, 10% glycerol. No preservative added.

Recombinant human ACE2.

ACE2 has direct effects on cardiac functions. It is expressed predominantly in vascular endothelial cells of the heart and kidney. ACE2 converts angiotensin I to angiotensin 1-9. ACE2 was also identified as a functional receptor for SARS coronavirus.

Applications:
Suitable for use in ELISA, Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA (Direct or Indirect): 1:2000-1:10, 000
Flow Cytometry: 1:1000
Western Blot: 1:2000-1:10, 000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Incubation time is 1 hour at RT is suggested.
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.