Comparison

Cyclin D1 (B cell CLL/lymphoma 1,B cell Leukemia 1,B Cell lymphoma 1 Protein,BCL-1 oncogene,CCND1 Protein,CCND1/FSTL3 fusion gene,CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene,CCND1/PTH fusion gene,cD1,Cyl 1,G1/S-speci

Item no. USB-172071
Manufacturer United States Biological
Amount 20 ug
Quantity options 20 ug 100 ug
Category
Type Antibody Monoclonal
Format Liquid
Applications WB, FC, IF, IP, IHC, ELISA
Clone SPM587
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Monkey (Cynomolgus, Simian)
Host Mouse
Isotype IgG2a
Purity Purified by Protein A/G affinity chromatography.
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Shipping Condition Cool pack
Available
Manufacturer - Type
Mab
Manufacturer - Category
Antibodies / Antibodies-Cyclin
Manufacturer - Isotype
IgG2a, k
Shipping Temperature
Blue Ice
Storage Conditions
-20°C
Grade
Affinity Purified
Form
Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 172071-AF.
EU Commodity Code
30021010
Immunogen
Human recombinant full length cyclin D1 protein. Cellular Localization: Nuclear
Specificity
Recognizes Cyclin D1. Species Crossreactivity: Human, Monkey, Mouse and Rat.
Description
Recognizes a protein of 36kD, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.5-1ug/million cells
Immunofluorescence: 1-2ug/ml
Western Blot: 0.5-1ug/ml
Immunoprecipitation: 1-2ug/500ug protein lysate
Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control:
T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. ~60% of mantle cell lymphomas and ~40% of breast carcinomas are positive.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Manufacturer - Full Name
Cyclin D1 (B cell CLL/lymphoma 1, B cell Leukemia 1, B Cell lymphoma 1 Protein, BCL-1 oncogene, CCND1 Protein, CCND1/FSTL3 fusion gene, CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene, CCND1/PTH fusion gene, cD1, Cyl 1, G1/S-specific cyclin-D1, Parat

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 20 ug
Available: In stock
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