CBL, phosphorylated (Y774) (E3 Ubiquitin-Protein Ligase CBL, Casitas B-lineage lymphoma proto-Oncogene, Proto-Oncogene c-Cbl, RING Finger Protein 55, Signal transduction Protein CBL, CBL, CBL2, RNF55)

Antibodies / Antibodies-Oncoproteins

-20°C

Supplied as a liquid PBS,  0.1% sodium azide,  50% glycerol.

Synthetic phosphopeptide derived from human Cbl around the phosphorylation site of Tyrosine 774.

Cbl (also designated the c-Cbl proto-oncogene, E3 ubiquitin-protein ligase CBL , Casitas B-lineage lymphoma proto-oncogene and RING finger protein 55) has been identified as the cellular homolog of the v-Cbl oncogene isolated from an NFS/N mouse that developed a pre-B cell lymphoma following infection with the replication-competent Cas Br-M murine leukemic virus. c-Cbl is expressed at relatively high levels in a wide range of hematopoietic tumor cell lines as well as in normal tissues such as thymus and testis. The c-Cbl gene product has been identified as a cytoplasmic protein with apparent DNA binding and dimerization domains characteristic of transcription factors. A single c-Cbl locus termed CBL2 has been mapped to human chromosome 11q23. This region of chromosome 11 is involved in translocations and deletions in a broad range of leukemias; c-Cbl has been found to be translocated from chromosome 11 in leukemias with either t(4; 11) or t(11; 14) abnormalities.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:50-1:200
Immunofluorescence: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.