ERAB (3-hydroxyacyl-CoA Dehydrogenase Type-2,17-beta-hydroxysteroid Dehydrogenase 10,17-beta-HSD 10,3-hydroxy-2-methylbutyryl-CoA Dehydrogenase,3-hydroxyacyl-CoA Dehydrogenase Type II,Endoplasmic Reticulum-Associated Amy

Antibodies / Antibodies-Enzymes, Dehydrogenase

-20°C

Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.

Synthetic peptide corresponding to amino acids 102-150 of Human ERAB.

beta-Amyloid is a neurotoxic peptide that is associated with the pathogenesis of Alzheimer’s disease. beta-Amyloid aggregates induce cell death of neurons through the disruption of cell membranes and the generation of reactive oxygen intermediates.These neurotoxic effects are also attributed to the interaction of beta-Amyloid with intracellular proteins, specifically ERAB, the endoplasmic reticulum-associated beta-Amyloid-binding protein. ERAB is characterized as a NAD+-dependent dehydrogenase that is constitutively expressed in tissues and overexpressed in neurons affected in Alzheimer’s disease. Cells overexpressing ERAB in vitro have been shown to be more sensitive to beta-Amyloid-induced stress, and blocking the activity of ERAB has been shown to inhibit this cell death, indicating that beta-Amyloid induced cell death is mediated by ERAB.

Applications:
Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.