FAK, phosphorylated (Y861) (Focal Adhesion Kinase 1, FADK 1, Focal Adhesion Kinase-Related nonKinase, FRNK, Protein Phosphatase 1 Regulatory Subunit 71, PPP1R71, Protein-Tyrosine Kinase 2, p125FAK, pp125FAK, PTK2, F

Antibodies / Antibodies-Tyrosine Kinases

-20°C

Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.

Synthetic phosphopeptide derived from human FAK around the phosphorylation site of Serine 861.

Focal adhesion kinase was initially identified as a major 125kDa substrate for the intrinsic protein tyrosine kinase activity of Src-encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds, and is activated and localized to the focal adhesions of spreading keratinocytes in culture.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.