hnRNP A2 (Heterogeneous Nuclear Ribonucleoproteins A2/B1, hnRNP A2/B1, HNRNPA2B1, HNRPA2B1)

Antibodies / Antibodies-Nuclear Proteins

-20°C

Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.

Synthetic peptide corresponding to the N-terminal of Human hnRNP A2.

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of poly-peptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are ~20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. The A/B subfamily of hnRNPs include A1, A2/B1, A3 and A0, and in Xenopus, hnRNP A1, A2 and A3 are ubiquitously expressed throughout development as well as in adult tissues. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The hnRNP A0 gene is distinct from the other A/B family members, and it encodes a low-abundance protein, which is implicated in mRNA stability.

Applications:
Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.