MAP-2 (Microtubule-Associated Protein 2, MAP2)

Antibodies / Antibodies-Microtubule-associated Proteins

-20°C

Supplied as a liquid 0.1M Tris-Glycine, pH 7.4, 150mM NaCl, 0.2% sodium azide, 50% glycerol

Recombinant full length Human MAP-2.

MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70kDa (MAP2c). MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 Immunocytochemistry: 1:100-200 Immunoprecipitation: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.