NEFL (Neurofilament Light Polypeptide, NF-L, 68kD Neurofilament Protein, Neurofilament triplet L Protein, NF68, NFL)

Antibodies / Antibodies-Neurofilament

-20°C

Supplied as a liquid PBS,  0.1% sodium azide,  50% glycerol.

Recombinant full length Human NEFL.

Neurofilament-L (for neurofilament light polypeptide, or NF-L), a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and actin filaments.

Applications:
Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.