NIPA (Nuclear-interacting Partner of ALK, Nuclear-interacting Partner of anaplastic lymphoma Kinase, hNIPA, Zinc Finger C3HC-Type Protein 1, ZC3HC1, NIPA, HSPC216)

Antibodies / Antibodies-Proteins

-20°C

Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.

Synthetic peptide corresponding to amino acids 318-372 of Human NIPA.

Entry into mitosis is essentially driven by cyclin B1 which is located in the cytoplasm throughout interphase, but accumulates in the nucleus just before mitosis occurs. Nuclear Interaction Partner of ALK (NIPA) plays a critical role in cyclin B1 regulation. NIPA is normally phosphorylated during G2 and M phases, resulting in an accumulation of cyclin B1. When NIPA sheds its attached phosphate, it binds to SCF to form the SCFNIPA complex, a member of the E3 ubiquitin ligases, which ubiquitinates cyclin B1, thereby targeting it to the proteosome for degradation.

Applications:
Suitable for use in Immunofluorescence, Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunofluorescence: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.