PARK7 (Protein DJ-1, Oncogene DJ1, Parkinson Disease Protein 7, PARK7)

Antibodies / Antibodies-Oncoproteins

-20°C

Supplied as a liquid PBS,  0.1% sodium azide,  50% glycerol.

Recombinant full length Human PARK7.

The DJ-1 gene encodes a highly-conserved protein which is implicated in a number of cell processes. DJ-1 was first identified as a novel oncogene that transformed mouse NIH/3T3 cells in cooperation with activated Ras. Additionally, DJ-1 was cloned in rat as SP22 or CAP-1, and found to be an infertilityrelated sperm protein whose expression is reduced in sperm treated with toxicants. DJ-1 also positively regulates the androgen receptor (AR) by forming a complex with PIASxalpha, a negative regulator of AR. The gene encoding human DJ-1 maps to chromosome 1p36.33-p36.12, a region identified as a hot spot of chromosome abnormalities in several tumor cells. Subsequently, mutations in the DJ-1 gene have been implicated in Parkinson’s disease, and loss of DJ-1 function leads to neurodegeneration.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:50-1:200
Immunocytochemistry: 1:50-1:200
Immunoprecipitation: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.