RANKL (Tumor Necrosis Factor Ligand Superfamily Member 11, Osteoclast Differentiation Factor, ODF, Receptor Activator of Nuclear Factor kappa-B Ligand, TNF-Related activation-induced cytokine, CD254, TNFSF11, OPGL, RANKL

Antibodies / Antibodies-RANK

-20°C

Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.

Synthetic peptide corresponding to amino acids 51-100 of Human RANKL.

Members of the tumor necrosis factor (TNF) receptor superfamily interact with signaling molecules of the TNF receptor-associated factor (TRAF) family to activate the NFκB and JNK pathways. RANK (receptor activator of NFκB) is a member of the TNFR family identified on dendritic cells. This type I membrane receptor is expressed in a broad range of tissues. The C-terminus of RANK is required for RANK to bind TRAF2, 5 and 6, and it is also necessary for stimulating NFκB activation. The ligand for this receptor, RANKL (also designated TRANCE, OPGL or ODF), is a type II transmembrane protein expressed primarily in lymphoid tissues and T cell lines. RANKL appears to be an important regulator of T cells and osteoclasts.

Applications:
Suitable for use in Immunofluorescence, Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunofluorescence: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.