SNRPE (B raf, B-raf, Braf, C76690, HYPT11, RUXE_HUMAN, Sm Protein E, Sm-E, Small Nuclear Ribonucleoprotein E, SmE, snRNP-E, snRNPE, snrpe,)

Antibodies / Antibodies-Nuclear Proteins

-20°C

Supplied as a liquid PBS,  0.1% sodium azide,  50% glycerol.

Recombinant full length Human SNRPE.

U1, U2, U5 and U4/U6 are small nuclear ribonucleoproteins (snRNPs) that comprise the spliceosome in eukaryotes. Each UsnRNP contains common Sm proteins B/B', D1, D2, D3, E, F and G. The Sm proteins pair up as D1-D2, B/B'-D3 and E-F-G to form RNA-free hetero-oligomers in the cytoplasm. Sm proteins aid in the cytoplasmic construction of the UsnRNPs by binding to a conserved Sm site on UsnRNA and forming a stable snRNP core complex. As a core protein to UsnRNP, the 11kDa Sm E associates with the entire U family of snRNAs including U1–U6. Autoantibodies produced in patients suffering from systemic lupus erythematosus react predominantly with Sm B/B', D1 and D3. The major linear epitope of these autoantibodies includes the C-terminal RG dipeptide repeats found in Sm D1 and D3.

Applications:
Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.