MECP2 (AUTSX 3,AUTSX3,DKFZp686A24160,Mbd 5,Mbd5,MECP 2,MeCP 2 Protein,MeCP-2 Protein,MeCP2,Mecp2,MECP2_HUMAN,Methyl CpG Binding Protein 2 (Rett Syndrome),Methyl CpG Binding Protein 2,Methyl-CpG-Binding Protein 2,MRX 16,MRX 79

Pab

Blue Ice

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.

Synthetic peptide corresponding to MeCP2.

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC) (By similarity).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.