CD40 (Tumor Necrosis Factor Receptor Superfamily Member 5, B Cell Surface Antigen CD40, Bp50, CD40L Receptor, TNFRSF5, GP39, HIGM1, IGM, IMD3, p50, T-BAM, TRAP)

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

(BALB/c x NZB)F1 mouse-derived lymphoma WEHI 231

CD40 is a 48kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39) expressed on activated T cells, is important in costimulation and immune regulation. This antibody has been reported to stimulate dendritic and B cell proliferation and block CD40-CD40L interactions.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: ≤1ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.