CD42a, gpIb, IX

Antibodies / Antibodies-CD Markers

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

Peripheral blood mononuclear cells.

CD42a cell surface antigen is a 22kD glycoprotein also known as the platelet glycoprotein gp Ib-IX complex. CD42a is expressed by platelets and megakaryocytes. Has been successfully used as a capture reagent for platelet-autoantibody complexes in the sera of patients presenting thrombocytopenia associated with antiphospholipid syndrome (Godeau et al. 1997).

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:50-1:200. Dilute in PBS. Use 50ul of diluted antibody per 1x10e6 platelets in 100ul of buffer.
Immunohistochemistry: frozen sections
Optimal working dilutions to be determined by researcher.

Immunohistochemistry Recommended Positive Control Tissue:
Bone marrow.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.