CD68 (DKFZp686M18236, GP110, Macrophage Antigen CD68 (Microsialin), Macrosialin, Scavenger Receptor Class D Member 1, SCARD1)

Antibodies / Antibodies-Receptors, Scavenger

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Purified Concanavalin A acceptor glycoprotein from P815 cell line

Macrosialin is a heavily glycosylated transmembrane protein of 87-115kD, which is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablization.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:50-1:100. 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required.
Immunohistochemistry (Paraffin): Heat induced epitope retrieval is recommended prior to staining paraffin section. Sodium citrate buffer, pH 6.0 is recommended.
Immunohistochemistry: Frozen sections
Western Blot: Non-reducing conditions recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.