Collagen Type I

Antibodies / Antibodies-Collagen

-20°C

Supplied as a liquid, 15mM sodium azide.

Full length, purified bovine native protein. Cellular Localization: Extracellular matrix.

The extracellular matrix is the material found in the extracellular environment of all tissues and organs. It consists of basement membranes and interstitial stroma. The composition of the extracellular framework of all vertebrates is dominated by a class of molecules known as collagens, each with unique features suited for its function and location. The collagens are proteins composed of three subunit polypeptides that can vary in length, which interact to form a triple helix. The molecular basis of the triple helix is provided by a repeated unique amino acid sequence (Gly-x-y) and the polypeptides generated are capable of assembly into fibrillar or other types of supramolecular assemblies which are deposited in the extracellular matrix. The number of distinct collagen types presently recognized is more than fourteen.

Applications:
Suitable for use in ELISA, Dot Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:2000-1:4000; The epitope recognized may be sensitive to routine formalin fixation and paraffin embedding. In testing of acetone-fixed frozen sections, strong staining of connective tissue fibers is seen.
Optimal dilutions to be determined by the researcher.

Recommended Tissue:
Human, porcine tongue and human, porcine tonsil

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.