Glucose Regulated Protein 75 (Grp75)

Mab

Bovine, Canine, Human, Monkey, Mouse, Porcine, Rat, Rabbit, Sheep, Xenopus

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

Grp75 isolated from human Molt-4 cells

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. One class of stress proteins, termed the Hsp70 family, is comprised of multiple members, all of which bind ATP in vitro, but which are localized within different intracellular compartments. These include: i) Hsc70 (or constitutive form) present within the cytosol/nucleus; ii) Hsp70 (inducible form) present within the cytosol/nucleus/nucleolus; iii) the constitutive glucose-regulated 78kD (or BiP) protein present within the lumen of the endoplasmic reticulum; and iv) the constitutive glucose regulated 75kD protein present within the mitochondrial matrix. Members of the Hsp70 family are thought to funtion as molecular chaperones (2), assisting in the folding of other proteins in various intracellular compartments (3). Grp75 is localized in the mitochondrial matrix, where, in concert with Hsp60, is thought to participate in the re-folding of proteins translocated into this organelle (4). Like its E. coli homolog DnaK (5), Grp75 possesses a cation-dependent ATPase activity (6, 7) thought to be central to its function as a chaperone.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot (Colorimetric): 1:1000. Sufficient for detection of Grp75 in 20ug of HeLa (heat shocked) cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:AP as the secondary antibody.
Immunohistochemistry: 1:50-1:200 using human breast cancer tissue.
Optimal dilutions to be determined by researcher.

Positive Controls:
HeLa Heat Shocked Cell Lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.