Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen)

Antibodies / Antibodies-Cancer Markers

-20°C

Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.

Synthetic peptide corresponding to the C-terminus of human Ki67. Cellular Localization: Nuclear.

Ki-67 is a nuclear protein, which is expressed in proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 1:300-1:600 for 20 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
Breast carcinoma and lymph node

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.