sRANKL (soluble RANK Ligand, Receptor Activator of NFkB Ligand)

Antibodies / Antibodies-RANK

-20°C

Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to 0.1-1mg/ml.

Highly pure (> 98%) recombinant human soluble RANKL

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution in conjunction with biotinylated anti-human sRANKL (R1076-05H) as a detection antibody, allows the detection of at least 2000-4000pg/well of recombinant human sRANKL.
Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human sRANKL is 1.5-3ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.