Comparison

Anti-Cytokeratin 14 Picoband Antibody

Item no. ABC-ABO10185
Manufacturer Abcepta
Amount 100 ug
Category
Type Antibody Primary
Format Lyophilized
Applications WB, IHC-P
Specific against other
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products KRT14, Keratin, K14, type I cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14
Available
Primary Accession
P02533
Application
WB, IHC-P
Clonality
Polyclonal
Gene ID
3861
Reactivity
H, M, Rat
Legend image 1
Western blot analysis of Cytokeratin 14 expression in rat brain extract (lane 1), NIH3T3 whole cell lysates (lane 2) and HEPG2 whole cell lysates (lane 3). Cytokeratin 14 at 60KD was detected using rabbit anti- Cytokeratin 14 Antigen Affinity purified polyclonal antibody (Catalog # ABO10185) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method .
Type image 1
WB
Dilution image 1
0.1-0.5 µg/ml
Legend image 2
Cytokeratin 14 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- Cytokeratin 14 Antigen Affinity purified polyclonal antibody (Catalog # ABO10185) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
Type image 2
IHC
Dilution image 2
0.5-1 µg/ml
Legend image 3
Cytokeratin 14 was detected in paraffin-embedded sections of human oesophagus squama cancer tissues using rabbit anti- Cytokeratin 14 Antigen Affinity purified polyclonal antibody (Catalog # ABO10185) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
Type image 3
IHC
Legend image 4
Cytokeratin 14 was detected in paraffin-embedded sections of human oesophagus squama cancer tissues using rabbit anti- Cytokeratin 14 Antigen Affinity purified polyclonal antibody (Catalog # ABO10185) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
Type image 4
IHC
Calculated MW
51561
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Description
Rabbit IgG polyclonal antibody for Keratin, type I cytoskeletal 14(KRT14) detection. Tested with WB, IHC-P in Human; Mouse; Rat.
Protein Function
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. .
Subcellular Localization
Cytoplasm. Nucleus. Expressed in both as a filamentous pattern.
Tissue Specificity
Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen. .
Protein Name
Keratin, type I cytoskeletal 14
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cytokeratin 14 (446-472aa RQIRTKVMDVHDGKVVSTHEQVLRTKN), identical to the related mouse and rat sequences.
Purification
Immunogen affinity purified.
Cross Reactivity
No cross reactivity with other proteins
Storage
At -20?C for one year. After r?Constitution, at 4?C for one month. It?Can also be aliquotted and stored frozen at -20?C for a longer time.Avoid repeated freezing and thawing.
Concentration (mg/ml)
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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