Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Primary |
Specific against |
other |
Format |
Affinity purified |
Applications |
WB, IHC |
Amount |
100 ul |
Host |
Rabbit |
Item no. |
ABC-AN1184 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Primary Accession |
P02452 |
Application |
IHC, WB |
Bio Background |
Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the must abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005). |
Bio References |
Byers PH (1989) Inherited disorders of collagen gene structure and expression. Am J MedGenet. 34(1):72-80.Cabral WA, Makareeva E, Colige A, Letocha AD, Ty JM, Yeowell HN, Pals G, Leikin S, MariniJC. (2005) Mutations near amino end of alpha1(I) collagen cause combined osteogenesisimperfecta/Ehlers-Danlos syndrome by interference with N-propeptide processing. J Biol Chem.2005 May 13; 280(19):19259-69. |
Clonality |
polyclonal |
Gene ID |
1277 |
Gene Name |
COL1A1 |
Subtitle |
Rabbit polyclonal antibody |
Reactivity |
H, M |
Legend image 1 |
Western blot of rat lung lysate showing specific immunolabeling of the ~ 140k collagen protein. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (red) that has formed fibrils in the extracellular matrix. |
Type image 2 |
IHC |
Dilution image 2 |
1:100 |
Calculated MW |
138941 |
Antigen Source |
human |
Target/Specificity |
Synthetic peptide corresponding to amino acid residues specific to the collagen 1, alpha 1 telopeptide conjugated to KLH. |
Antibody Specificity |
Specific for the ~ 140 kDa telopeptide portion of the collagen I α1polypeptide. The antibody works well for immunohistochemistry on paraformaldehyde-fixedsections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90º C in 10mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehydefixedfibrotic mouse lung tissue, the antibody recognizes mature collagen I that has formedfibrils in the extracellular matrix. |
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All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.