LEF1 Antibody (N-term)

Synthetic Peptide

This gene encodes a transcription factor belonging to a
family of proteins that share homology with the high mobility group
protein-1. The protein encoded by this gene can bind to a
functionally important site in the T-cell receptor-alpha enhancer,
thereby conferring maximal enhancer activity. This transcription
factor is involved in the Wnt signaling pathway, and it may
function in hair cell differentiation and follicle morphogenesis.
Mutations in this gene have been found in somatic sebaceous tumors.
This gene has also been linked to other cancers, including
androgen-independent prostate cancer. Alternative splicing results
in multiple transcript variants.

Polyclonal

51176

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Fluorescent confocal image of A431 cell stained with LEF1 Antibody (N-term)(Cat#AP12048a).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with LEF1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). LEF1 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.

1:10~50

WB

Anti-LEF1 Antibody (N-term) at 1:2000 dilution + Jurkat whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 44 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

WB

Q9QXN1; P27782; NP_057353.1

Rabbit Ig

10-37

This LEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-37 amino acids from the N-terminal region of human LEF1.