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PIAS2 (PIASx1/2) Antibody (C-term)

PIAS2 (PIASx1/2) Antibody (C-term)

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Isotype	Ig
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications	IHC-P
Amount	400 ul
Host	Rabbit
Item no.	ABC-AP1247a
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	O75928
Antigen Type	Synthetic Peptide
Application	IHC-P
Bio Background	PIASX functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. PIASX binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha.
Bio References	Sakurai, T., et al., J. Biol. Chem. 279(15):15505-15514 (2004). Arora, T., et al., J. Biol. Chem. 278(24):21327-21330 (2003). Wu, S., et al., Oncogene 22(3):351-360 (2003). Liu, B., et al., Proc. Natl. Acad. Sci. U.S.A. 95(18):10626-10631 (1998). Wu, L., et al., Mech. Dev. 65 (1-2), 3-17 (1997).
Clonality	Polyclonal
Clone/Animal Names	RB0701
Gene ID	9063
Gene Name	PIAS2
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Type image 1	IHC-P
Dilution image 1	1:50~100
Other Accession	Q6AZ28
Predicted	Rat
Isotype	Rabbit Ig
Calculated MW	68240
Antigen Region	518-547
Antigen Source	HUMAN
Target/Specificity	This PIAS2 (PIASx1/2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 518-547 amino acids from the C-terminal region of human PIAS2 (PIASx1/2).
NCBI Accession	NP_004662.2; NP_775298.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 6/6/2024