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SOX2 Antibody (N-term)

SOX2 Antibody (N-term)

Item no.	ABC-AP2048D
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	SOX2, Transcription factor SOX-2
Available
Primary Accession	P48431
Antigen Type	Synthetic Peptide
Application	WB, IHC-P, IF, FC
Bio Background	The intronless gene for SOX2 encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.
Bio References	Remenyi, A., et al., Genes Dev. 17(16):2048-2059 (2003). Wiebe, M.S., et al., J. Biol. Chem. 278(20):17901-17911 (2003). Fantes, J., et al., Nat. Genet. 33(4):461-463 (2003). Schepers, G.E., et al., Dev. Cell 3(2):167-170 (2002). Kamachi, Y., et al., Trends Genet. 16(4):182-187 (2000).
Clonality	Polyclonal
Clone/Animal Names	RB14026
Gene ID	6657
Gene Name	SOX2
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Immunofluorescence analysis of SOX2 Antibody (N-term) (Cat.#AP2048d) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of SOX2 Antibody (N-term) in 293 cell line lysates (35ug/lane). SOX2(arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of SOX2 (arrow) using rabbit polyclonal SOX2 Antibody (N-term) (Cat.#AP2048d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies).
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 antibody (N-term) (Cat.#AP2048d), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 4	IHC-P
Dilution image 4	1:10~50
Legend image 5	Flow cytometric analysis of NCI-H292 cells using SOX2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 5	FC
Dilution image 5	1:10~50
Other Accession	Q6EJB7; O42569; P48432; Q6P0E1; P48430; Q2PG84; P53783; O00570; O57401; Q2Z1R2; Q6DGL6; P54231
Predicted	Zf, C, M, X, Sh
Isotype	Rabbit Ig
Calculated MW	34310
Antigen Region	89-119
Antigen Source	HUMAN
Target/Specificity	This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-119 amino acids from the N-terminal region of human SOX2.
NCBI Accession	NP_003097.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 11/6/2025

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