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XAF1 Antibody (C-term)

XAF1 Antibody (C-term)

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Isotype	Ig
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, IF
Amount	400 ul
Host	Rabbit
Item no.	ABC-AP20645c
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	Q6GPH4
Application	IF, WB
Bio Background	Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti- caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha- induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.
Bio References	Liston P., et al.Nat. Cell Biol. 3:128-133(2001). Chung S.K., et al.Gastroenterology 132:2459-2477(2007). Ota T., et al.Nat. Genet. 36:40-45(2004). Mural R.J., et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Bechtel S., et al.BMC Genomics 8:399-399(2007).
Clonality	Polyclonal
Clone/Animal Names	RB49525
Gene ID	54739
Gene Name	XAF1
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Fluorescent image of Hela cells stained with XAF1 Antibody (C-term)(Cat#AP20645c). AP20645c was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Type image 1	IF
Dilution image 1	1:25
Legend image 2	Western blot analysis of lysates from human liver and spleen tissue lysate(from left to right), using XAF1 Antibody (C-term)(Cat. #AP20645c). AP20645c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Type image 2	WB
Dilution image 2	1:1000
Isotype	Rabbit Ig
Calculated MW	34626
Antigen Source	HUMAN
Target/Specificity	This XAF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 235-269 amino acids from the C-terminal region of human XAF1.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 6/6/2024