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USP25 Antibody

USP25 Antibody

Item no.	ABC-ASC11789
Manufacturer	Abcepta
Amount	100 ug
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Applications	WB, IF, ELISA, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Primary Accession	Q9UHP3
Application	WB, IHC-P, IF, E
Bio Background	USP25 (ubiquitin specific peptidase 25), also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response (1). It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform (2). The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function. USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling (3, 4).
Bio References	Valero R, Marfany G, Gonzalez-Angulo O, et al. USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2. Genomics 1999; 62:395-405. Valero R, Bayes M, Francisca Sanchez-Font M, et al. Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25. Genome Biol. 2001; 2: RESEARCH0043. Zhong B, Liu X, Wang X, et al. Negative regulation of IL-17-mediated signaling and inflammation by the ubiquitin-specific protease USP25. Nat. Immunol. 2012; 13:1110-7. Zhong B, Liu X, Wang X, et al. Ubiquitin-specific protease 25 regulates TLR4-dependent innate immune responses through deubiquitination of the adaptor protein TRAF3. Sci. Signal. 2013; 6:ra35.
Clonality	Polyclonal
Gene ID	29761
Gene Name	USP25
Reactivity	H, M
Legend image 1	Western blot analysis of USP25 in mouse brain tissue lysate with USP25 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.
Type image 1	WB
Legend image 2	Immunohistochemistry of USP25 in human brain tissue with USP25 antibody at 5 µg/mL.
Type image 2	IHC
Legend image 3	Immunofluorescence of USP25 in human brain tissue with USP25 antibody at 20 µg/mL.
Type image 3	IF
Other Accession	NP_037528; 50312666
Isotype	IgG
Calculated MW	122218
Antigen Source	homo sapiens
Target/Specificity	USP25; USP25 antibody is human and mouse reactive. Multiple isoforms of USP25 are known to exist.
Purification	USP25 antibody is affinity chromatography purified via peptide column.
Reconstitution & Storage	USP25 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
Antibody Form	Liquid
Application Notes	USP25 antibody can be used for detection of USP25 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.
Positive Control	Mouse Brain Tissue Lysate;
Buffer	USP25 Antibody is supplied in PBS containing 0.02% sodium azide.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 10/2/2025

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