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Alpha B Crystallin Antibody

Alpha B Crystallin Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	RPE
Amount	Inquiry
Item no.	ABC-ASM10097-RPE-200
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	P02511
Reactivity	H, Rat, B
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 3A10.H4 (ASM10097). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody (ASM10097) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Alpha B Crystallin Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of Alpha B Crystallin protein using Mouse Anti-Alpha B Crystallin Monoclonal Antibody, Clone 3A10-C9 (ASM10097). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha B Crystallin Monoclonal Antibody (ASM10097) at 1:50 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Type image 2	WB
Dilution image 2	1:2000
Other Accession	NP_001876.1
Isotype	IgG1
Target/Specificity	Detects ~20kDa (predicted mol.weight is ~21kDa). Does not cross-react with αA-crystallin, βL-crystallin, ΒH-crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Immunogen	Native Alpha B Crystallin
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASM10097-RPE-200-datasheet.pdf

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Delivery expected until 7/4/2024