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mGluR1/5 glutamate receptor Antibody

mGluR1/5 glutamate receptor Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	Streptavidin
Amount	Inquiry
Item no.	ABC-ASM10253-STR-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	P31424
Reactivity	H, M, Rat
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 glutamate receptor Monoclonal Antibody, Clone S75-3 (ASM10253). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-mGluR1/5 glutamate receptor Monoclonal Antibody (ASM10253) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X.
Type image 1	ICC/IF
Dilution image 1	1:1000
Legend image 2	Western Blot analysis of Rat Brain Membrane showing detection of ~130 kDa mGluR5 protein using Mouse Anti-mGluR5 Monoclonal Antibody, Clone S75-33 (ASM10253). Lane 1: Molecular Weight (MW) Ladder. Lane 2: Rat Brain Membrane. Load: 10 µg. Block: 5% milk. Primary Antibody: Mouse Anti-mGluR5 Monoclonal Antibody (ASM10253) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~130 kDa.
Type image 2	WB
Dilution image 2	1:1000
Other Accession	NP_058708.1
Isotype	IgG2A
Target/Specificity	Detects ~130kDa (both mGluR1 and mGluR5).
Immunogen	Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASM10253-STR-100-datasheet.pdf

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Delivery expected until 7/4/2024