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Kir6.1 Antibody

Kir6.1 Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	ATTO 680
Amount	Inquiry
Item no.	ABC-ASM10323-A680-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	Q63664
Reactivity	H, M, Rat
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 (ASM10323). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody (ASM10323) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Kir6.1 Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Rat Brain showing detection of ~45 kDa Kir6.1 protein using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 (ASM10323). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody (ASM10323) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~45 kDa. Other Band(s): ~100 kDa.
Type image 2	WB
Dilution image 2	1:1000
Other Accession	NP_058795.3
Isotype	IgG2A
Target/Specificity	Detects ~45kDa.
Immunogen	Fusion protein amino acids 306-424 (Cytoplasmic C-terminus) of rat Kir6.1
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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ABC-ASM10323-A680-100-datasheet.pdf

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Delivery expected until 6/6/2024