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Dibromo-tyrosine Antibody

Dibromo-tyrosine Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	ATTO 594
Amount	Inquiry
Item no.	ABC-ASM10335-A594-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 9F12 (ASM10335). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody (ASM10335) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A, C, E, G) - Untreated. (B, D, F, H) - Cells cultured overnight with 50 µM H2O2. (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alex Fluor 633 F-Actin stain. (E, F) Dibromo-tyrosine Antibody. (G, H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Type image 1	ICC/IF
Dilution image 1	1:50
Legend image 2	Western Blot analysis of 3, 5-Dibromotyrosine-BSA Conjugate showing detection of 67 kDa 3, 5-Dibromotyrosine-BSA using Mouse Anti-3, 5-Dibromotyrosine Monoclonal Antibody, Clone 9F12 (ASM10335). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: Nitrosylated-BSA. Lane 4: Dibromotyrosine-BSA. Load: 1 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-3, 5-Dibromotyrosine Monoclonal Antibody (ASM10335) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western Blot analysis of Human Cervical Cancer cell line (HeLa) showing detection of 3, 5-Dibromotyrosine-BSA using Mouse Anti-3, 5-Dibromotyrosine Monoclonal Antibody, Clone 9F12 (ASM10335). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-3, 5-Dibromotyrosine Monoclonal Antibody (ASM10335) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
Type image 3	WB
Isotype	IgG1
Target/Specificity	Specific for 3, 5-Dibromo-tyrosine. Does not cross-react with Nitrotyrosine.
Immunogen	Synthetic 3, 5-Dibromotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Delivery expected until 6/6/2024