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WIPI2 Antibody

WIPI2 Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	PerCP
Amount	Inquiry
Item no.	ABC-ASM10525-PCP-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	Q9Y4P8
Reactivity	H
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WIPI2 Polyclonal Antibody (ASM10525). Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody (ASM10525) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WIPI2 Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of ~49.4 kDa WIPI2 protein using Rabbit Anti-WIPI2 Polyclonal Antibody (ASM10525). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody (ASM10525) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~49.4 kDa.
Type image 2	WB
Dilution image 2	1:1000
Other Accession	NP_001028690.1
Target/Specificity	Detects ~55 kDa.
Immunogen	Synthetic peptide from the C-terminal of Human WIPI2
Purification	Peptide Affinity Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASM10525-PCP-100-datasheet.pdf

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Delivery expected until 7/4/2024