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RAT IgG2a isotype control Biotin

RAT IgG2a isotype control Biotin

Manufacturer	Abcepta
Category	Antibodies Isotype Controls
Type	Isotype Control
Specific against	other
Amount	50 ug
Item no.	ABC-ASR1132
eClass 6.1	32160702
eClass 9.0	42039027
Available
Bio Background	RAT IgG2a isotype control is used in flow cytometry, western blot and ELISA and differentiate between immunoglobulin classes and subclasses. Isotype controls allow for the genetic variations or differences in the constant regions of the heavy and light chains. In Rat there are six relevant heavy chain isotypes and two light chain isotypes: heavy chain a - IgA, ? - IgG 1, 2a, 2b, 2c and µ - IgM, light chain ? and ?.
Clonality	Monoclonal
Subtitle	Monoclonal IgG2a , Biotin
Concentration (mg/ml)	1.0 mg/mL by UV absorbance at 280 nm
Conjugate	Biotin
Application Note	ELISA 1:2000-1:20, 000; FlowCytometry 1:1000-1:5000
FP Value	2-8 moles Biotin per mole of Rat IgG2a
Physical State	Liquid (sterile filtered)
Host Isotype	IgG2a
Species of Origin	Rat
PSC Type	Protein
Stabilizer	None
Preservative	0.01% (w/v) Sodium Azide
Shipping Condition	Wet Ice
Purity	RAT IgG2a isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1, IgG2b, IgG3 , IgM , and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Light and heavy chain composition has been confirmed.
Storage Condition	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASR1132-datasheet.pdf

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Amount: 50 ug

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Delivery expected until 7/4/2024