Comparison

Anti-Mouse IgG1 (Peroxidase Conjugated) Pre-adsorbed Secondary Antibody

Item no. ABC-ASR2717
Manufacturer Abcepta
Amount 1 mg
Category
Type Antibody Secondary
Specific against other
Host Goat
Conjugate/Tag HRP
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Available
Bio Background
HRP Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Clonality
Polyclonal
Subtitle
Goat Polyclonal, Peroxidase (Horseradish)
Reactivity
M
Immunogen
Mouse IgG1 heavy chain
Concentration (mg/ml)
1.0 mg/mL by UV absorbance at 280 nm
Conjugate
Peroxidase (Horseradish)
Application Note
ELISA 1:20, 000-1:100, 000; Western Blot 1:2, 000-1:20, 000; Immunochemistry 1:1, 000-1:5, 000
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Target Species
Mouse
Physical State
Lyophilized
Target Isotype
IgG1
Host Isotype
IgG
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Shipping Condition
Ambient
Purity
Anti-Mouse IgG1 HRP secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse Serum and Mouse IgG. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Storage Condition
Store vial antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 mg
Available: In stock
available

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