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Cynomolgus CD3D & CD3E Heterodimer Protein

Cynomolgus CD3D & CD3E Heterodimer Protein

Item no.	CT032-C0323H-20
Manufacturer	Sino Biological
Amount	20 ug
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins
Format	Human Cell
Specific against	Monkey (Cynomolgus, Simian)
Host	Human
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	CT032-C0323H
Similar products	CD3D & CD3E
Available
Molecule	CD3D & CD3E
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD3E extracellular domain (Met 1-Asp 117) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD3D extracellular domain (Met 1-Ala 105) with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the CD3E&CD3D heterodimer was purified.
Expressed Host	Human Cells
Purity	(50.3+48.5) % as determined by SDS-PAGE
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Gln 22 & Phe 22
Molecule Mass	The recombinant heterodimer of cynomolgus CD3E&CD3D comprises 679 (346+333) amino acids and has a calculated molecular mass of 76.7 (39.1+ 37.6) KDa. The apparent molecular mass of cyno CD3E&CD3D heterodimer is approximately 52 & 43 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4
Amino acid sequence	Met1-Asp117 & Met1-Ala105
TAG	C-human IgG1-Fc & His & C-human IgG1-Fc & Flag

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CT032-C0323H-20-datasheet.pdf

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Amount: 20 ug

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