Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Primary |
Specific against |
other |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB, FC, IHC-P |
Amount |
100 ul |
Host |
Rabbit |
Item no. |
ABC-AW5309-U100 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Primary Accession |
P37840 |
Antigen Type |
Synthetic Peptide |
Application |
FC, WB, IHC-P |
Bio Background |
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. |
Bio References |
Ueda K., et al.Proc. Natl. Acad. Sci. U.S.A. 90:11282-11286(1993).Campion D., et al.Genomics 26:254-257(1995).Ueda K., et al.Biochem. Biophys. Res. Commun. 205:1366-1372(1994).Xia Y., et al.Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.Touchman J.W., et al.Genome Res. 11:78-86(2001). |
Clonality |
Polyclonal |
Gene ID |
6622 |
Gene Name |
SNCA |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, Rat |
Legend image 1 |
Western blot analysis of lysates from human brain, mouse brain tissue lysate (from left to right), using SNCA Antibody (C-term)(Cat. #AW5309). AW5309 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
AW5309 staining SNCA in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. |
Type image 2 |
IHC-P |
Dilution image 2 |
1:25 |
Legend image 3 |
Flow cytometric analysis of HeLa cells using SNCA Antibody (C-term)(green, Cat#AW5309) compared to an isotype control of rabbit IgG(blue). AW5309 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody. |
Type image 3 |
FC |
Dilution image 3 |
1:25 |
Predicted |
M |
Isotype |
Rabbit Ig |
Calculated MW |
14460 |
Antigen Region |
92-125 |
Antigen Source |
HUMAN |
Target/Specificity |
This SNCA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 92-125 amino acids from the C-terminal region of human SNCA. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.