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Anti-TNF Alpha Antibody

Anti-TNF Alpha Antibody

Item no.	ANTI-A282930-100
Manufacturer	Antibodies.com
Amount	100 ug
Category	Antibodies Immunohistochemistry Western Blot ELISA Primary Antibodies Immunostaining Functional Assays
Type	Antibody Primary
Format	Lyophilized
Applications	WB, IHC, ELISA, FA
Specific against	Mouse (Murine, Mus musculus)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Unconjugated
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Available
Storage Conditions	Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Product Description	Rabbit polyclonal antibody to TNF alpha.
Clonality	Polyclonal
Immunogen	Recombinant mouse TNF alpha.
Concentration	1 mg/ml after reconstitution.
Manufacturer - Formulation	Supplied in Phosphate Buffered Saline.
Recommended dilutions	ELISA: 0.5 - 2 µg/ml, Functional Assays: 0.01 - 0.015 µg/ml, WB: 0.1 - 0.2 µg/ml
Manufacturer - Specificity	This antibody recognises mouse Tumour Necrosis Factor alpha (TNF–alpha), a 17.3kDa multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. TNF-alpha is involved in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. This Rabbit anti Mouse TNF-alpha antibody can be used to neutralize the effects of mouse TNF-alpha.
Reconstitution	Reconstitute with 0.1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. For functional studies do not add sodium azide.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 11/13/2025

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