Item no. |
B-BNC040520-100 |
Manufacturer |
Biotium
|
Amount |
100 uL |
Quantity options |
100 uL
500 uL
|
Category |
|
Type |
Antibody Primary |
Format |
Liquid |
Applications |
WB, IHC |
Clone |
MUC1/520 |
Specific against |
Human (Homo sapiens) |
Host |
Mouse |
Isotype |
IgG2a Kappa |
Conjugate/Tag |
CF405S |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Breast carcinoma-associated antigen DF3; CA15-3; Carcinoma-associated mucin Episialin; Epithelial Membrane Antigen; H23AG; KL-6; MAM6; MUC-1; MUC-1/SEC; MUC-1/X; MUC1-alpha; MUC1-beta; MUC1-CT; MUC1-NT; MUC1/ZD; Mucin 1 cell surface associated; Mucin-1 subunit beta; Peanut-reactive urinary mucin; PEM; PEMT; Polymorphic epithelial mucin; PUM; Tumor-associated epithelial membrane antigen |
Shipping condition |
Room temperature |
Available |
|
Manufacturer - Type |
Primary |
Manufacturer - Applications |
IHC, FFPE (verified)|WB (verified) |
Manufacturer - Category |
Antibodies |
Manufacturer - Targets |
CD227|EMA|Episialin|Mucin 1 |
Manufacturer - Conjugate / Tag |
CF405S |
Shipping Temperature |
Room temperature |
Storage Conditions |
Store at 2 to 8°C|Protect fluorescent conjugates from light |
Molecular Weight |
265-400 kDa |
Manufacturer - Research Area |
Cancer|Mucins |
Description |
In Western blotting, it recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors. |
Product origin |
Animal |
Ingredient of biological origin |
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells. |
Special handling for PI and label |
Protect from light |
Undated stability guarantee in PI |
2 years |
Stability at RT during shipping (protected from light) |
Stable at room temperature or 37°C (98°F) for 7 days. |
UNSPSC Commodity |
41116161 |
UNSPSC Commodity Title |
Primary and secondary antibodies for multiple methodology immunostaining detection application |
Classified or regulated chemicals |
0.05% sodium azide (CAS 26628-22-8) |
Concentration |
0.1 mg/mL |
Storage buffer |
PBS, 0.1% BSA, 0.05% azide |
Dated shelf life printed on label |
Guaranteed for at least 24 months from date of receipt when stored as recommended |
Immunogen (Secondaries, for anti tag only) |
Human milk-fat globule membranes (HMFGM) |
Clonality |
Monoclonal |
Regulatory Status |
For research use only (RUO) |
Human Gene Symbol |
MUC1 |
Unigene |
89603 |
Positive Control |
MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma. |
Antibody target cellular localization |
Plasma membrane |
Cell tissue expression |
Epithelial cells|Mammary gland |
Tumor expression |
Breast cancer |
Antibody application notes |
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.1-0.2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user |
Antibody Number |
#0520 |
Verified Antibody Applications |
IHC (FFPE) (verified)|WB (verified) |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.