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MART-1 / Melan-A(M2-7C10 + M2-9E3 + A103), CF640R conjugate, 0.1mg/mL

MART-1 / Melan-A(M2-7C10 + M2-9E3 + A103), CF640R conjugate, 0.1mg/mL

Item no.	B-BNC400700-500
Manufacturer	Biotium
Amount	500 uL
Quantity options	100 uL 500 uL
Category	Oncology Antibodies By Cell Type Melanoma Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	IHC
Clone	M2-7C10 M2-9E3 A103
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus), Dog (Canine, Canis lupus familiaris)
Host	Mouse
Isotype	IgG
Conjugate/Tag	CF640R
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA
Shipping Condition	Room temperature
Available
Manufacturer - Type	Primary
Manufacturer - Applications	IHC, FFPE (verified)
Manufacturer - Category	Antibodies
Manufacturer - Isotype	IgG' s
Manufacturer - Targets	MART-1\|Melan-A
Manufacturer - Conjugate / Tag	CF640R
Manufacturer - Host	Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Shipping Temperature	Room temperature
Storage Conditions	Store at 2 to 8°C\|Protect fluorescent conjugates from light
Molecular Weight	20-22 kDa (doublet)
Manufacturer - Research Area	Cancer
Description	This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb cocktail labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin	Animal
Ingredient of biological origin	Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Special handling for PI and label	Protect from light
Undated stability guarantee in PI	2 years
Stability at RT during shipping (protected from light)	Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity	41116161
UNSPSC Commodity Title	Primary and secondary antibodies for multiple methodology immunostaining detection application
Classified or regulated chemicals	0.05% sodium azide (CAS 26628-22-8)
Concentration	0.1 mg/mL
Storage buffer	PBS, 0.1% BSA, 0.05% azide
Dated shelf life printed on label	Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)	Recombinant hMART-1 protein (A103; M2-7C10; M2-9E3)
Clonality	Monoclonal
Regulatory Status	For research use only (RUO)
Human Gene Symbol	MLANA
Unigene	154069
Positive Control	SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas
Antibody target cellular localization	Golgi apparatus
Cell tissue expression	Melanocytes
Tumor expression	Melanoma
Antibody application notes	Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody\|Immunofluorescence: 0.5-1 ug/mL\|Immunohistology formalin-fixed 0.5-1 ug/mL\|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes\|Flow Cytometry 0.5-1 ug/million cells/0.1 mL\|Western blotting 0.5-1.0 ug/mL\|Optimal dilution for a specific application should be determined by user
Manufacturer - Antibody Reactivity	MART-1\|Melan-A

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

B-BNC400700-500-datasheet.pdf

B-BNC400700-500-safety-datasheet.pdf