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Melanoma Marker (MART-1 + gp100)(DT101+ BC199 + HMB45), CF640R conjugate, 0.1mg/mL

Melanoma Marker (MART-1 + gp100)(DT101+ BC199 + HMB45), CF640R conjugate, 0.1mg/mL

Item no.	B-BNC400703-500
Manufacturer	Biotium
Amount	500 uL
Quantity options	100 uL 500 uL
Category	Oncology Neuroscience Aging & Neurological Disorders Antibodies By Cell Type Hematology Leukemia Lymphoma Melanoma Primary Antibodies By Organ Skin Cancer Renal Cancer
Type	Antibody Primary
Format	Liquid
Applications	IHC
Clone	DT101 BC199 HMB45
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 Kappa
Conjugate/Tag	CF640R
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A; PMEL17
Shipping Condition	Room temperature
Available
Manufacturer - Type	Primary
Manufacturer - Applications	IHC, FFPE (verified)
Manufacturer - Category	Antibodies
Manufacturer - Targets	Melanoma cells
Manufacturer - Conjugate / Tag	CF640R
Manufacturer - Host	Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Shipping Temperature	Room temperature
Storage Conditions	Store at 2 to 8°C\|Protect fluorescent conjugates from light
Molecular Weight	20-22 kDa (doublet) (MART-1); 90-100 kDa (gp100)
Manufacturer - Research Area	Cancer
Description	This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Product origin	Animal
Ingredient of biological origin	Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Special handling for PI and label	Protect from light
Undated stability guarantee in PI	2 years
Stability at RT during shipping (protected from light)	Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity	41116161
UNSPSC Commodity Title	Primary and secondary antibodies for multiple methodology immunostaining detection application
Classified or regulated chemicals	0.05% sodium azide (CAS 26628-22-8)
Concentration	0.1 mg/mL
Storage buffer	PBS, 0.1% BSA, 0.05% azide
Dated shelf life printed on label	Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)	Recombinant hMART-1 protein (DT101 & BC199); Extract of pigmented melanoma metastases from lymph nodes (HMB45)
Clonality	Monoclonal
Regulatory Status	For research use only (RUO)
Human Gene Symbol	MLANA & SILV
Unigene	154069 ; 95972
Positive Control	SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.
Antibody target cellular localization	Cytoplasmic
Cell tissue expression	Melanocytes
Tumor expression	Melanoma
Antibody application notes	Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody\|Immunohistochemistry (formalin-fixed): 0.5-1.0 ug/mL for 30 minutes at RT\|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes\|Optimal dilution for a specific application should be determined by user
Manufacturer - Antibody Reactivity	Melanoma cells

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

B-BNC400703-500-datasheet.pdf

B-BNC400703-500-safety-datasheet.pdf